Faculty of Nursing – University of Kufa Official Site – Faculty of Nursing
Instructor Assistant Prof. Dr. Ali Abdel-Zahra Al-Faham published a scientific research in an international journal entitled
(The Effect of Ascorbic Acid and Selenium intake on serum Cortisol in Rats Under Restraint Stress )
Over the recent years ,vitamin C and selenium have been a clear line of defense against the effects of stress.The goal of the study is to indicate the effect of restraint stress in rats on the serum levels of cortisol, the role of vitamin C and selenium supplement in stress relief by determining their effect on serum cortisol . forty Wistar rats were divided randomly into four equal groups (n=10), all the animal except negative control exposed to restraint stress for six hours a day . the first group were supplemented with vitamin C in a dose
of (50 mg/kg bw/day) orally, the second included given selenium in a dose of (0.02 µg/kg bw/day) orally,the control included ten rats . Blood was collected on 20th days, and serum cortisol were measured by enzyme-linked immunosorbent assay (ELISA). Results showed that supplement of rats with vitamin C had high significant increasein serum cortisol in stressed rat (14.65 ± 5) compared to those with no stress (8.77± 4) which constitute the negative control . The study also revealed that supplement of rats with vitamin C ,Selenium and (vitamin C + Selenium) have highly significantly increased serum cortisol level to be (10.42 ±3.78), (10.29 ± 4.54) and (12.77 ± 3.57) respectively compared to stressed rats . It is concluded that chronicstress restriction can result in cumulative initiation of cortisol secretion in rats. Intake of vitamin C can relieve stress by increasing decreasing cortisol .
To view the research, please visit the link below.
